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ROBO1 antibody

Orthogonal
Anti-ROBO1 antibody used in Western Blot (WB). GTX114103
Anti-ROBO1 antibody used in Western Blot (WB). GTX114103
Anti-ROBO1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114103
Anti-ROBO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114103
Anti-ROBO1 antibody used in Western Blot (WB). GTX114103

Cat. No. GTX114103

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Chicken
Package
100 μl ($399),
25 μl ($169)
See all ROBO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

181 kDa. ( Note )

Positive Control

MDA-Mb-361 , HCC1937 LROBO1 (Lentivirus infection carry human ROBO1) , HeLa membrane fraction

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human ROBO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166472

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

roundabout guidance receptor 1 , DUTT1 , SAX3

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ROBO1 antibody used in Western Blot (WB). GTX114103

GTX114103 WB Image

Orthogonal

HeLa whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody (GTX114103) diluted at 1:1000.

Anti-ROBO1 antibody used in Western Blot (WB). GTX114103

GTX114103 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody (GTX114103) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-ROBO1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114103

GTX114103 ICC/IF Image

ROBO1 antibody detects ROBO1 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ROBO1 stained by ROBO1 antibody (GTX114103) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-ROBO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114103

GTX114103 IHC-P Image

ROBO1 antibody detects ROBO1 protein at cytosol on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ROBO1 antibody (GTX114103) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ROBO1 antibody used in Western Blot (WB). GTX114103

GTX114103 WB Image

Sample (15 μg of whole cell lysate)
A: MDA-Mb-361
B: HCC1937 Lx ( Lentivirus infection, carry contol vector)
C: HCC1937 LROBO1 (Lentivirus infection, carry human ROBO1)
GTX114103 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Yang T et al. Cell Rep 2018; 24 (10) : 2694-2708.e6 miR-92 Suppresses Robo1 Translation to Modulate Slit Sensitivity in Commissural Axon Guidance.
Application : ICC/IF
Reactivity : Chicken
Perez-Ortiz AC et al. Oncotarget 2017; 8 (63) : 106454-106467
Perez-Ortiz A et al. Oncotarget 2017; (Epub) pharmacogenetics of response to neoadjuvant * treatment for locally advanced breast cancer.
Application : WB
Reactivity : Human
Choi YJ et al. Pathol Oncol Res 2014; 20 (3) : 517-9 Down-regulation of ROBO2 expression in prostate cancers.
Application : IHC-P
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169